3 research outputs found
Changes in the Qualitative Composition of the milk of Holstein Cows During Summer Chronic Heat Stress
Seasonal summer heat stress leads to significant economic losses, resulting in a drop in milk yield in dairy cows and a deterioration in milk quality. The purpose of this study was to determine the changes in some qualitative parameters of milk in Holstein cows during chronic heat stress on one of the largest commercial dairy farms Ukraine (50Β°49β²14β³ N, 31Β°49β²23β³ E). Five multiparous cows of medium lactation with a milk yield of about 30 kg per day were randomly selected into the reference (in the spring, at the end of May) and the experimental group (in the summer, at the end of August). Milk samples were taken from cows during the morning milking. Qualitative analysis of milk included the identification of milk solids-not-fat, density, mass fraction of lactose, fat, protein and minerals, as well as the freezing point, electrical conductivity and active acidity using ultrasonic method. Animals were kept in naturally ventilated barns. The total mixed single-type balanced diet consisting of corn silage and concentrates that the cows received remained unchanged. The sampling of milk from cows of the experimental group was preceded by a continuous 26-day hot period, during which the maximum daily values of the temperature and humidity index did not fall below 72 units. The results showed that in the milk of the animals of the experimental group there was a significant decrease in the content of milk solids-not-fat, the mass fraction of fat and protein (P0.05). The mass fraction of lactose and minerals tended to increase. It should be noted that the average daily milk yield of dairy cows in the herd in spring and summer was almost at the same level. In conclusion, the results of the study showed that high summer temperatures lead to a deterioration in the quality of milk in Holstein cows. Despite the decrease in milk density and freezing point, these figures met the requirements of the standard. Organoleptic indicators of milk, electrical conductivity and active acidity of milk did not change in hot weather, their value indicated the naturalness of milk. The mass fraction of milk fat, which undergoes the greatest change under the influence of seasonal heat stress, is one of the most valuable components of milk, which has a direct effect on nutritional value and purchase price of raw milk. Therefore, a further deeper study of the fatty acid composition of milk using the method of chromato-mass spectrometry will provide valuable data necessary to search for possible herd management strategies to maintain high milk quality under conditions of seasonal heat stress
ΠΠΌΡΡΡ ΠΆΠΈΡΠ½ΠΈΡ ΠΊΠΈΡΠ»ΠΎΡ Π² Π»ΡΠΏΡΠ΄Π°Ρ ΠΌΠ΅Π·Π΅Π½Ρ ΡΠΌΠ½ΠΈΡ ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ ΠΊΠ»ΡΡΠΈΠ½ ΠΊΡΠ»ΡΡΡΡΠΈ ΠΆΠΈΡΠΎΠ²ΠΎΡ ΡΠΊΠ°Π½ΠΈΠ½ΠΈ
The content of fatty acids in the lipids of mesenchymal stem cells of dog adipose tissue culture was studied. Mesenchymal stem cells of dog adipose tissue culture were obtained by culturing the primary material in a CO2 incubator with a content of 5 % CO2, at a temperature of 37 Β°C in DMEM medium with the addition of 10β15 % fetal bovine serum and 1 % antibiotic-antimycotic. When the confluency of the monolayer reached 70β80 %, the cells were transferred to a suspension and subcultivated in order to reduce the heterogeneity of the culture and obtain a sufficient amount of biological material. The lipids of the obtained stem cells were analyzed for the content of fatty acids by the method of thin-layer gas-liquid chromatography. Determination of the content of lipids of fatty acids in FSK of a cat was carried out by the Folch method. A mixture of fatty acid methyl esters was analyzed on a Trace GC Ultra gas chromatograph with a flame ionization detector on a capillary column SPTM β2560, 100 m x 0.25 mm ID, 0.20 ΞΌm film (Supelco). Identification of fatty acids was carried out using a standard sample of Supelco 37 Π‘omponent FAME Mix. Quantitative assessment of the LC spectrum was carried out by the method of normalization of the peak planes of methylated LC derivatives and their content was determined as a percentage of the total content of all LC. The conducted study of the content of fatty acids in lipids made it possible to reveal certain features of the lipid metabolism of mesenchymal stem cells cultured in dog adipose tissue. A high content of oleic acid, characteristic of cells resistant to apoptosis and with high proliferative potential, was determined; a high ratio of unsaturated linoleic to saturated stearic acid (Π‘18:1/Π‘18.0), which reflects the high activity of the stearoyl-coenzyme-desaturase enzyme and, indirectly, the active state of the Wnt/Ξ²-catenin signaling pathway; inability to lengthen the chain of saturated fatty acids; lack or low activity of de novo synthesis of omega-6 polyunsaturated fatty acids. 18 fatty acids were found in the composition of lipids of fetal stem cells of a cat, of the saturated ones - the most palmitic acid (33.70 Β± 0.02 %), of the monounsaturated ones β oleic acid (21.63 Β± 0.03 %), of the polyunsaturated ones β linoleic acid (6.45 Β± 0.07 %). The least amount of cis-,11,14-eicosadienoic acid (0.04 Β± 0.01 %) was found in the composition of cell lipids. The total amount of saturated fatty acids in dog mesenchymal stem cell lipids was 65.65 Β± 0.02 %), unsaturated fatty acids β 34.35 Β± 0.02 %. Monoene fatty acids were determined in the amount of 24.46 Β± 0.02 %, and polyene β 9.89 Β± 0.02 %. The ratio index of polyunsaturated fatty acids Ο 3 to Ο 6 is 0.40. Lipids of mesenchymal stem cells of adipose tissue culture were characterized by a lower content of monoene unsaturated fatty acids 24.46 Β± 0.02; (P < 0.05), with a higher content of Ο3 fatty acids 3.04 Β± 0.02 %; (P < 0.05), with a lower content of Ο6 fatty acids 6.86 Β± 0.02 %; (P < 0.05) in contrast to lipids of red bone marrow stem cells.ΠΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½ΠΎ Π²ΠΌΡΡΡ ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ Π² Π»ΡΠΏΡΠ΄Π°Ρ
ΠΌΠ΅Π·Π΅Π½Ρ
ΡΠΌΠ½ΠΈΡ
ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ ΠΊΡΠ»ΡΡΡΡΠΈ ΠΆΠΈΡΠΎΠ²ΠΎΡ ΡΠΊΠ°Π½ΠΈΠ½ΠΈ ΡΠΎΠ±Π°ΠΊΠΈ. ΠΠ΅Π·Π΅Π½Ρ
ΡΠΌΠ½Ρ ΡΡΠΎΠ²Π±ΡΡΠΎΠ²Ρ ΠΊΠ»ΡΡΠΈΠ½ΠΈ ΠΊΡΠ»ΡΡΡΡΠΈ ΠΆΠΈΡΠΎΠ²ΠΎΡ ΡΠΊΠ°Π½ΠΈΠ½ΠΈ ΡΠΎΠ±Π°ΠΊΠΈ ΠΎΡΡΠΈΠΌΡΠ²Π°Π»ΠΈ ΡΠ»ΡΡ
ΠΎΠΌ ΠΊΡΠ»ΡΡΠΈΠ²ΡΠ²Π°Π½Π½Ρ ΠΏΠ΅ΡΠ²ΠΈΠ½Π½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΡΠ°Π»Ρ Π² Π‘Π2 ΡΠ½ΠΊΡΠ±Π°ΡΠΎΡΡ Π· Π²ΠΌΡΡΡΠΎΠΌ 5 % Π‘Π2, Π·Π° ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠΈ 37 Β°Π‘ Ρ ΡΠ΅ΡΠ΅Π΄ΠΎΠ²ΠΈΡΡ DMEM Π· Π΄ΠΎΠ΄Π°Π²Π°Π½Π½ΡΠΌ 10β15 % ΡΠ΅ΡΠ°Π»ΡΠ½ΠΎΡ ΡΠΈΡΠΎΠ²Π°ΡΠΊΠΈ Π²Π΅Π»ΠΈΠΊΠΎΡ ΡΠΎΠ³Π°ΡΠΎΡ Ρ
ΡΠ΄ΠΎΠ±ΠΈ ΡΠ° 1 % Π°Π½ΡΠΈΠ±ΡΠΎΡΠΈΠΊΠ°-Π°Π½ΡΠΈΠΌΡΠΊΠΎΡΠΈΠΊΠ°. ΠΠΎΠ»ΠΈ ΠΊΠΎΠ½ΡΠ»ΡΠ΅ΡΠ½ΡΡΡΡ ΠΌΠΎΠ½ΠΎΡΠ°ΡΡ ΡΡΠ³Π°Π»Π° 70β80 %, ΠΊΠ»ΡΡΠΈΠ½ΠΈ ΠΏΠ΅ΡΠ΅Π²ΠΎΠ΄ΠΈΠ»ΠΈ Π² ΡΡΡΠΏΠ΅Π½Π·ΡΡ ΡΠ° ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΡΠ±ΠΊΡΠ»ΡΡΠΈΠ²ΡΠ²Π°Π½Π½Ρ Π· ΠΌΠ΅ΡΠΎΡ Π·Π½ΠΈΠΆΠ΅Π½Π½Ρ Π³Π΅ΡΠ΅ΡΠΎΠ³Π΅Π½Π½ΠΎΡΡΡ ΠΊΡΠ»ΡΡΡΡΠΈ ΡΠ° ΠΎΡΡΠΈΠΌΠ°Π½Π½Ρ Π΄ΠΎΡΡΠ°ΡΠ½ΡΠΎΡ ΠΊΡΠ»ΡΠΊΠΎΡΡΡ Π±ΡΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΡΠ°Π»Ρ. ΠΡΠΏΡΠ΄ΠΈ ΠΎΡΡΠΈΠΌΠ°Π½ΠΈΡ
ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΡΠ²Π°Π»ΠΈ Π½Π° Π²ΠΌΡΡΡ ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠΎΠ½ΠΊΠΎΡΠ°ΡΠΎΠ²ΠΎΡ Π³Π°Π·ΠΎΡΡΠ΄ΠΈΠ½Π½ΠΎΡ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΡΡ. ΠΠΈΠ·Π½Π°ΡΠ΅Π½Π½Ρ Π²ΠΌΡΡΡΡ Π»ΡΠΏΡΠ΄ΡΠ² ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ Π€Π‘Π ΠΊΠΎΡΠ° ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π€ΠΎΠ»ΡΠ°. Π‘ΡΠΌΡΡ ΠΌΠ΅ΡΠΈΠ»ΠΎΠ²ΠΈΡ
Π΅ΡΡΡΡΠ² ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ Π°Π½Π°Π»ΡΠ·ΡΠ²Π°Π»ΠΈ Π½Π° Π³Π°Π·ΠΎΠ²ΠΎΠΌΡ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΡ Trace GC Ultra Π· ΠΏΠΎΠ»ΡΠΌβΡΠ½ΠΎ-ΡΠΎΠ½ΡΠ·Π°ΡΡΠΉΠ½ΠΈΠΌ Π΄Π΅ΡΠ΅ΠΊΡΠΎΡΠΎΠΌ Π½Π° ΠΊΠ°ΠΏΡΠ»ΡΡΠ½ΡΠΉ ΠΊΠΎΠ»ΠΎΠ½ΡΡ SPTMβ2560, 100 m Γ 0,25 mm ID, 0,20 ΞΌm film (Supelco). ΠΠ΄Π΅Π½ΡΠΈΡΡΠΊΠ°ΡΡΡ ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π·Π° Π΄ΠΎΠΏΠΎΠΌΠΎΠ³ΠΎΡ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΠΎΠ³ΠΎ Π·ΡΠ°Π·ΠΊΠ° Supelco 37 Π‘omponent FAME Mix. ΠΡΠ»ΡΠΊΡΡΠ½Ρ ΠΎΡΡΠ½ΠΊΡ ΡΠΏΠ΅ΠΊΡΡΡ ΠΠ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π½ΠΎΡΠΌΡΠ²Π°Π½Π½Ρ ΠΏΠ»ΠΎΡΠΈΠ½ ΠΏΡΠΊΡΠ² ΠΌΠ΅ΡΠΈΠ»ΡΠΎΠ²Π°Π½ΠΈΡ
ΠΏΠΎΡ
ΡΠ΄Π½ΠΈΡ
ΠΠ Ρ Π²ΠΈΠ·Π½Π°ΡΠ°Π»ΠΈ ΡΡ
Π½ΡΠΉ Π²ΠΌΡΡΡ Ρ Π²ΡΠ΄ΡΠΎΡΠΊΠ°Ρ
Π²ΡΠ΄ ΡΡΠΌΠ°ΡΠ½ΠΎΠ³ΠΎ Π²ΠΌΡΡΡΡ ΡΡΡΡ
ΠΠ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½Π΅ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ Π²ΠΌΡΡΡΡ ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ Π² Π»ΡΠΏΡΠ΄Π°Ρ
Π΄Π°Π»ΠΎ ΠΌΠΎΠΆΠ»ΠΈΠ²ΡΡΡΡ Π²ΠΈΡΠ²ΠΈΡΠΈ ΠΏΠ΅Π²Π½Ρ ΠΎΡΠΎΠ±Π»ΠΈΠ²ΠΎΡΡΡ Π»ΡΠΏΡΠ΄Π½ΠΎΠ³ΠΎ ΠΎΠ±ΠΌΡΠ½Ρ ΠΌΠ΅Π·Π΅Π½Ρ
ΡΠΌΠ½ΠΈΡ
ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ ΠΊΡΠ»ΡΡΡΡΠΈ ΠΆΠΈΡΠΎΠ²ΠΎΡ ΡΠΊΠ°Π½ΠΈΠ½ΠΈ ΡΠΎΠ±Π°ΠΊΠΈ. ΠΠΈΠ·Π½Π°ΡΠ΅Π½ΠΎ Π²ΠΈΡΠΎΠΊΠΈΠΉ Π²ΠΌΡΡΡ ΠΎΠ»Π΅ΡΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ, Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ½ΠΈΠΉ Π΄Π»Ρ ΠΊΠ»ΡΡΠΈΠ½, ΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΠΈΡ
Π΄ΠΎ Π°ΠΏΠΎΠΏΡΠΎΠ·Ρ ΡΠ° Π· Π²ΠΈΡΠΎΠΊΠΈΠΌ ΠΏΡΠΎΠ»ΡΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΈΠΌ ΠΏΠΎΡΠ΅Π½ΡΡΠ°Π»ΠΎΠΌ; Π²ΠΈΡΠΎΠΊΠ΅ ΡΠΏΡΠ²Π²ΡΠ΄Π½ΠΎΡΠ΅Π½Π½Ρ Π½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΎΡ Π»ΡΠ½ΠΎΠ»Π΅Π²ΠΎΡ Π΄ΠΎ Π½Π°ΡΠΈΡΠ΅Π½ΠΎΡ ΡΡΠ΅Π°ΡΠΈΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ (Π‘18:1/Π‘18.0), ΡΠΊΠ΅ Π²ΡΠ΄ΠΎΠ±ΡΠ°ΠΆΠ°Ρ Π²ΠΈΡΠΎΠΊΡ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΡΡΠ΅Π°ΡΠΎΠ»-ΠΊΠΎΠ΅Π½Π·ΠΈΠΌ-Π΄Π΅ΡΠ°ΡΡΡΠ°Π·ΠΈ ΡΠ° ΠΎΠΏΠΎΡΠ΅ΡΠ΅Π΄ΠΊΠΎΠ²Π°Π½ΠΎ β Π°ΠΊΡΠΈΠ²Π½ΠΈΠΉ ΡΡΠ°Π½ Wnt/Ξ²-ΠΊΠ°ΡΠ΅Π½ΡΠ½ ΡΠΈΠ³Π½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ»ΡΡ
Ρ; Π½Π΅Π·Π΄Π°ΡΠ½ΡΡΡΡ Π΄ΠΎ ΠΏΠΎΠ΄ΠΎΠ²ΠΆΠ΅Π½Π½Ρ Π»Π°Π½ΡΡΠ³Π° Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ; Π²ΡΠ΄ΡΡΡΠ½ΡΡΡΡ Π°Π±ΠΎ Π½ΠΈΠ·ΡΠΊΡ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ ΡΠΈΠ½ΡΠ΅Π·Ρ de novo ΠΎΠΌΠ΅Π³Π°-6 ΠΏΠΎΠ»ΡΠ½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ. Π£ ΡΠΊΠ»Π°Π΄Ρ Π»ΡΠΏΡΠ΄ΡΠ² ΡΠ΅ΡΠ°Π»ΡΠ½ΠΈΡ
ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ ΠΊΠΎΡΠ° Π²ΠΈΡΠ²Π»Π΅Π½ΠΎ 18 ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ, Π· Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
β Π½Π°ΠΉΠ±ΡΠ»ΡΡΠ΅ ΠΏΠ°Π»ΡΠΌΡΡΠΈΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ (33,70 Β± 0,02 %), Π· ΠΌΠΎΠ½ΠΎΠ½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
β ΠΎΠ»Π΅ΡΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ (21,63 Β± 0,03 %), Π· ΠΏΠΎΠ»ΡΠ½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
β Π»ΡΠ½ΠΎΠ»Π΅Π²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ (6,45 Β± 0,07 %). ΠΠ°ΠΉΠΌΠ΅Π½ΡΠ΅ Ρ ΡΠΊΠ»Π°Π΄Ρ Π»ΡΠΏΡΠ΄ΡΠ² ΠΊΠ»ΡΡΠΈΠ½ Π²ΠΈΡΠ²Π»Π΅Π½ΠΎ ΡΡΡ-,11,14-Π΅ΠΉΠΊΠΎΠ·Π°Π΄ΡΡΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ (0,04 Β± 0,01 %). Π‘ΡΠΌΠ°ΡΠ½Π° ΠΊΡΠ»ΡΠΊΡΡΡΡ Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ Ρ Π»ΡΠΏΡΠ΄Π°Ρ
ΠΌΠ΅Π·Π΅Π½Ρ
ΡΠΌΠ½ΠΈΡ ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ ΡΠΎΠ±Π°ΠΊΠΈ ΡΡΠ°Π½ΠΎΠ²ΠΈΠ»Π° 65,65Β± 0,02%), Π½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ β 34,35 Β± 0,02 %. ΠΠΎΠ½ΠΎΡΠ½ΠΎΠ²Ρ ΠΆΠΈΡΠ½Ρ ΠΊΠΈΡΠ»ΠΎΡΠΈ Π²ΠΈΠ·Π½Π°ΡΠ΅Π½ΠΎ Ρ ΠΊΡΠ»ΡΠΊΠΎΡΡΡ 24,46Β± 0,02%, Π° ΠΏΠΎΠ»ΡΡΠ½ΠΎΠ²Ρ β 9,89Β± 0,02%. ΠΠ½Π΄Π΅ΠΊΡ ΡΠΏΡΠ²Π²ΡΠ΄Π½ΠΎΡΠ΅Π½Π½Ρ ΠΏΠΎΠ»ΡΠ½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ Ο 3 Π΄ΠΎ Ο 6 ΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ 0,40. ΠΡΠΏΡΠ΄ΠΈ ΠΌΠ΅Π·Π΅Π½Ρ
ΡΠΌΠ½ΠΈΡ
ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ ΠΊΡΠ»ΡΡΡΡΠΈ ΠΆΠΈΡΠΎΠ²ΠΎΡ ΡΠΊΠ°Π½ΠΈΠ½ΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΠ²Π°Π»ΠΈΡΡ Π½ΠΈΠΆΡΠΈΠΌ ΡΠΌΡΡΡΠΎΠΌ ΠΌΠΎΠ½ΠΎΡΠ½ΠΎΠ²ΠΈΡ
Π½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ 24,46 Β± 0,02; (P < 0,05), Π±ΡΠ»ΡΡΠΈΠΌ Π²ΠΌΡΡΡΠΎΠΌ Ο3 ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ 3,04 Β± 0,02 %; (P < 0,05), ΠΌΠ΅Π½ΡΠΈΠΌ Π²ΠΌΡΡΡΠΎΠΌ Ο6 ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ 6,86 Β± 0,02 %; (P < 0,05) Π½Π° ΠΏΡΠΎΡΠΈΠ²Π°Π³Ρ Π»ΡΠΏΡΠ΄Π°ΠΌ ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ ΠΊΡΠ»ΡΡΡΡΠΈ ΡΠ΅ΡΠ²ΠΎΠ½ΠΎΠ³ΠΎ ΠΊΡΡΡΠΊΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠΎΠ·ΠΊΡ